two hybrid system Search Results


two hybrid system  (TaKaRa)
97
TaKaRa two hybrid system
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
Two Hybrid System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
two hybrid system - by Bioz Stars, 2026-05
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yeast two-hybrid screening  (Hybrigenics sa)
90
Hybrigenics sa yeast two-hybrid screening
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
Yeast Two Hybrid Screening, supplied by Hybrigenics sa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yeast two-hybrid screening - by Bioz Stars, 2026-05
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yeast two-hybrid system  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies yeast two-hybrid system
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
Yeast Two Hybrid System, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yeast two-hybrid system - by Bioz Stars, 2026-05
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membrane yeast two-hybrid screen assay  (Dualsystems Biotech)
90
Dualsystems Biotech membrane yeast two-hybrid screen assay
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
Membrane Yeast Two Hybrid Screen Assay, supplied by Dualsystems Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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membrane yeast two-hybrid screen assay - by Bioz Stars, 2026-05
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checkmatetm mammalian twohybrid system  (Promega)
90
Promega checkmatetm mammalian twohybrid system
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
Checkmatetm Mammalian Twohybrid System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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checkmatetm mammalian twohybrid system - by Bioz Stars, 2026-05
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checkmate mammalian two-hybrid system  (Promega)
90
Promega checkmate mammalian two-hybrid system
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
Checkmate Mammalian Two Hybrid System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/checkmate mammalian two-hybrid system/product/Promega
Average 90 stars, based on 1 article reviews
checkmate mammalian two-hybrid system - by Bioz Stars, 2026-05
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hybrid two- and zero-dimensional quantum dot photodetectors  (Quantum Dot Inc)
90
Quantum Dot Inc hybrid two- and zero-dimensional quantum dot photodetectors
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
Hybrid Two And Zero Dimensional Quantum Dot Photodetectors, supplied by Quantum Dot Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
hybrid two- and zero-dimensional quantum dot photodetectors - by Bioz Stars, 2026-05
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yeast two-hybrid screen  (Curagen Inc)
90
Curagen Inc yeast two-hybrid screen
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
Yeast Two Hybrid Screen, supplied by Curagen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yeast two-hybrid screen - by Bioz Stars, 2026-05
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bacterial two-hybrid system  (Euromedex)
90
Euromedex bacterial two-hybrid system
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
Bacterial Two Hybrid System, supplied by Euromedex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bacterial two-hybrid system/product/Euromedex
Average 90 stars, based on 1 article reviews
bacterial two-hybrid system - by Bioz Stars, 2026-05
90/100 stars
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yeast two-hybrid experiment  (Blackwell Science Ltd)
90
Blackwell Science Ltd yeast two-hybrid experiment
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
Yeast Two Hybrid Experiment, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
yeast two-hybrid experiment - by Bioz Stars, 2026-05
90/100 stars
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bacterial adenylate cyclase two hybrid system kit  (Euromedex)
90
Euromedex bacterial adenylate cyclase two hybrid system kit
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
Bacterial Adenylate Cyclase Two Hybrid System Kit, supplied by Euromedex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bacterial adenylate cyclase two hybrid system kit/product/Euromedex
Average 90 stars, based on 1 article reviews
bacterial adenylate cyclase two hybrid system kit - by Bioz Stars, 2026-05
90/100 stars
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two-hybrid tests  (Hybrigenics sa)
90
Hybrigenics sa two-hybrid tests
(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) <t>Yeast</t> <t>two-hybrid</t> assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).
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(A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) Yeast two-hybrid assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).

Journal: bioRxiv

Article Title: Unstructured regions differentially modulate the activation of RBOHD and RBOHH

doi: 10.64898/2026.03.31.715192

Figure Lengend Snippet: (A,B) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHD variants carrying progressive deletions in the UR1 region (DΔ60, DΔ69, DΔ83, DΔ100, DΔ121, DΔ134, DΔ155, DΔ170) or in the EF-hand domain (DΔ181, DΔ253). (C,D) Ca 2+ -induced ROS production in HEK293T cells expressing 3×FLAG-RBOHH variants with deletions in the UR1 region (HΔ11, HΔ23, HΔ34, HΔ56, HΔ69, HΔ94, HΔ109). (E) Yeast two-hybrid assay using the whole N-terminus of RBOHD (NT RBOHD) and RBOHH (NT RBOHH), together with negative control (CTRL). Growth on DDO confirms the presence of both AD and BD plasmids. whereas growth on TDO + 3-AT and QDO indicates protein–protein interaction (F,G) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing RBOHD UR1 truncation variants. (H) Schematic representation of chimeric RBOHD–RBOHH variants (χ1, χ2, χ3). Domains derived from RBOHD are shown in pink, and domains derived from RBOHH are shown in cyan. (I) Ca 2+ -induced ROS production of chimeric RBOHD–RBOHH variants. (J) BIK1-induced and BIK1 + Ca 2+ -induced ROS production in HEK293T cells expressing chimeric RBOHD–RBOHH variants. (K) ROS production in leaf discs from plants overexpressing RBOHD or the chimera χ3 following flg22 treatment. ROS activity is expressed as a percentage of the maximal response of wild-type protein in all panels (For panel I RBOHD was considered as the wild-type). For Ca 2+ -induced conditions (A–D and I), luminescence was recorded every 1 min before ionomycin addition (highlighted in the graphs) and every 30 s thereafter. For BIK1-dependent conditions (F, G, J) and flg22 treatment (K), luminescence was recorded every 1 min before and after stimulation. Data represent mean ± SEM from N = 5 technical replicates. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test on the area under the ROS production curve. Different letters indicate statistically significant differences (P < 0.05).

Article Snippet: Yeast two-hybrid assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech).

Techniques: Expressing, Y2H Assay, Negative Control, Derivative Assay, Activity Assay, Comparison